Protection of Inonotus hispidus (Bull.) P. Karst. against Chronic Alcohol-Induced Liver Injury in Mice via Its Relieving Inflammation Response

Alcoholic liver disease (ALD) can be induced by excessive alcohol consumption, and has a worldwide age-standardized incidence rate (ASIR) of approximately 5.243%. Inonotus hispidus (Bull.) P. Karst. (IH) is mushroom with pharmacological effects. In ALD mice, the hepatoprotective effects IH were investigated. strongly ameliorated alcohol-induced pathological changes in liver, including structures its function-related indices. Intestinal microbiota serum metabolomics analysis showed that altered associated anti-inflammatory metabolites. According to results obtained from Western blot, immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), downregulated levels pro-inflammation factors interleukin (IL)-1β, IL-6 tumor necrosis factor-α (TNF-α), enhanced expressions peroxisome proliferator-activated receptor alpha (PPARα) 15-hydroxprostaglandin dehydrogenase (15-PGDH), inhibited phosphorylated activation Janus kinase (JAK) 1 signal transducer activator transcription (STAT) 3, confirming hepatoprotection against damage via anti-inflammation. This study provides experimental evidence for chronic ALD.